A Poly-D-Mannose Synthesized by a One-Pot Method Exhibits Anti-Biofilm, Antioxidant, and Anti-Inflammatory Properties In Vitro

In this study, D-mannose was used to synthesize poly-D-mannose using a one-pot method. The molecular weight, degree of branching, monosaccharide composition, total sugar content, and infrared spectrum were determined. addition, we evaluated the safety bioactivity including anti-pathogen biofilm, antioxidant, anti-inflammatory activity. results showed that mixture four components with different weights. weight first three larger than 410,000 Da, fourth 3884 Da. branching 0.53. content 97.70%, composed only mannose. spectra possessed characteristic groups polysaccharides. Poly-D-mannose no cytotoxicity or hemolytic activity at concentration range from 0.125 mg/mL 8 mg/mL. had best inhibition effect on Salmonella typhimurium 2 (68.0% ± 3.9%). Escherichia coli O157:H7 not obvious, biofilm reduced by 37.6% 2.9% For Staphylococcus aureus Bacillus cereus, biofilms low concentration; however, rates 33.7% 6.4% 47.5% 4%, respectively. scavenging ability free radicals. It DPPH, highest rate 74.0% 2.8%, followed hydroxyl radicals, 36.5% 1.6%; superoxide anion radicals ABTS lowest, 10.1% 2.1% 16.3% 0.9%, lipopolysaccharide (LPS)-stimulated macrophages, decreased secretion nitric oxide (NO) reactive oxygen species (ROS), down-regulated expression tumor necrosis factor-alpha (TNF-α) interleukin-6 (IL-6). Therefore, it can be concluded prepared in research is safe has certain biological Meanwhile, provides new idea for development novel prebiotics food feed industries active ingredients pharmaceutical production future.